Human AHSA1 antibody

The Co-chaperone AHA1 (Activator of HSP90 ATPase-1) is proposed to function as an ATPase activating protein. May affect a step in the endoplasmic reticulum to Golgi trafficking. It expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.

Product Specifications

Product Name Alternative

Activator of Hsp90 ATPase-1 (AHA1, 19-337), AHA1, AHSA1, Activator of Hsp90 ATPase protein 1, Hsp90 co-chaperone AHA1, Activator of Hsp90 ATPase-1 Activator of 90 kDa heat shock protein ATPase homolog 1, Activator of heat shock 90kDa protein ATPase homolog 1, C14orf3, HSPC322, p38

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human AHA1 (19-337aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT3E9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

AHA1, Activator of Hsp90 ATPase-1, AHA1, Activator of Hsp90 ATPase-1 (AHA1, 19-337), Activator of Hsp90 ATPase protein 1, Hsp90 co-chaperone AHA1, Activator of Hsp90 ATPase-1 Activator of 90 kDa heat shock protein ATPase homolog 1, Activator of heat shock 90kDa protein ATPase homolog 1, AHA 1, AHSA 1, AHSA1, C14orf3, HSPC322, p38, ATGA0388-10 µg, ATGA0388-20 µg, ATGA0388-50 µg, ATGA0388-100 µg, ATGA0388-250 µg, ATGA0388-500 µg, ATGA0388-1 mg, ATGA0388-010, ATGA0388-020, ATGA0388-050, ATGA0388-100, ATGA0388-250, ATGA0388-500, ATGA0388-01M

References & Citations

Meyer P., et al. (2004) EMBO. 23 (6) : 1402-1410. ; ; Siligardi G., et al. (2004) J Biol Chem. 270 (50) : 51989-51998.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_036243

Uniprot Accession Number

O95433

WB Description

The Recombinant Human AHA1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AHA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : AHA1 Recombinant protein ; ; Lane 2. : MCF-7 cell lysate ; ; Lane 3. : PC-3 cell lysate ; ; Lane 4. : 293T cell lysate ; ; Lane 5. : HeLa cell lysate

IF Description

ICC/IF analysis of AHA1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AHA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of AHA1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AHA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0388-IF2.jpg}