Human LMW-PTP/ACP1 antibody

Low molecular weight phosphotyrosine protein phosphatase, also known as ACP1, is an enzyme that catalyzes the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol. And, It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate.

Product Specifications

Product Name Alternative

Acid phosphatase 1 soluble isoform b, Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase, Acid phosphatase 1, soluble isoform b Acid phosphatase 1 soluble, Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Acid phosphatase of erythrocyte, PAP2, Protein tyrosine phosphatase, PTPase, Purple acid phosphatase, Red cell acid phosphatase 1.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACP1 (1-158aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT5C11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

LMW-PTP, ACP1, Acid phosphatase 1 soluble isoform b, Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase, Acid phosphatase 1, soluble isoform b Acid phosphatase 1 soluble, Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Acid phosphatase of erythrocyte, PAP2, Protein tyrosine phosphatase, PTPase, Purple acid phosphatase, Red cell acid phosphatase 1., ATGA0387-10 µg, ATGA0387-20 µg, ATGA0387-50 µg, ATGA0387-100 µg, ATGA0387-250 µg, ATGA0387-500 µg, ATGA0387-1 mg, ATGA0387-10, ATGA0387-20, ATGA0387-50, ATGA0387-100, ATGA0387-250, ATGA0387-500, ATGA0387-1

References & Citations

Junien C., et al. (1979) Hum Genet. 48 (1) : 17-21.; ; Dissing J., et al. (1992) Biochim Biophys Acta. 1121: 261-268.; ; Lazaruk KD., et al. (1993) Biochem Biophys Res Commun. 196: 440-446.; ; Bryson GL., et al. (1995) Genomics. 30: 133-140.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_009030

Uniprot Accession Number

P24666

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : Jurkat cell lysate ; ; Lane 3. : TF-1 cell lysate ; ; Lane 4. : NIH/3T3 cell lysate ; ; Lane 5. : HepG2 cell lysate

IF Description

ICC/IF analysis of ACP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .