Human Gankyrin/PSMD10 antibody

26S proteasome non-ATPase regulatory subunit 10 is an enzyme that in humans is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.

Product Specifications

Product Name Alternative

Proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1, ankyrin repeat protein p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, PSMD10.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PSMD10 (1-226aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1F4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PSMD10, proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1, ankyrin repeat protein p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, PSMD10., ATGA0386-10 µg, ATGA0386-20 µg, ATGA0386-50 µg, ATGA0386-100 µg, ATGA0386-250 µg, ATGA0386-500 µg, ATGA0386-1 mg, ATGA0386-10, ATGA0386-20, ATGA0386-50, ATGA0386-100, ATGA0386-250, ATGA0386-500, ATGA0386-1

References & Citations

Hori T., et al. (1998) Gene. 216 (1) : 113-122.; ; Kaneko T., et al. (2009) Cell. 137: 914-925.; ; Wang J., et al. (2009) Cytogenet Genome Res. 125: 87-97.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Ubiquitination

NCBI Accession Number

NP_002805

Uniprot Accession Number

O75832

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD10 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : A549 cell lysate ; ; Lane 2. : PC3 cell lysate ; ; Lane 3. : K562 cell lysate ; ; Lane 4. : HepG2 cell lysate ; ; Lane 5. : HeLa cell lysate ; ; Lane 6. : Jurkat cell lysate

IF Description

ICC/IF analysis of PSMD10 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMD10 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .