Human LSM5 antibody

LSM5, also known as YER146W, is a 91 amino acid protein belonging to the snRNP Sm family. Localized to the nucleus, LSm5 has been found to play a role in the formation and function of the U6 snRNP. Specifically, LSm5 binds to the 3' end of U6 snRNA and facilitates U4/U6 duplex formation. Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

Product Specifications

Product Name Alternative

U6 snRNA-associated Sm-like protein LSm5 isoform a, LSM5 homolog, u6 small nuclear RNA associated (S.cerevisiae), YER146W

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human LSM5 (1-91aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT25F8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

LSM5, u6 snRNA-associated Sm-like protein LSm5 isoform a, LSM5 homolog, u6 small nuclear RNA associated (S.cerevisiae), YER146W, ATGA0385-10 µg, ATGA0385-20 µg, ATGA0385-50 µg, ATGA0385-100 µg, ATGA0385-250 µg, ATGA0385-500 µg, ATGA0385-1 mg, ATGA0385-10, ATGA0385-20, ATGA0385-50, ATGA0385-100, ATGA0385-250, ATGA0385-500, ATGA0385-1

References & Citations

Salgado-Garrido J., et al. (1999) EMBO J. 18: 3451-3462.; ; Achsel T., et al. (1999) EMBO J. 18: 5789-5802.; ; Friesen WJ., et al. (2000) J Biol Chem. 275: 26370-26375.; ; Eystathioy T., et al. (2002) Arthritis Rheum. 46: 726-734.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_036454

Uniprot Accession Number

Q9Y4Y9

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LSM5 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : K562 cell lysate ; ; Lane 3. : Jurkat cell lysate

IF Description

ICC/IF analysis of LSM5 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LSM5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .