Human S100A4 antibody

S100 calcium-binding protein A4 (S100A4) is member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Specifications

Product Name Alternative

S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human S100A4 (1-101aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1C3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

S100A4, S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a, ATGA0384-10 µg, ATGA0384-20 µg, ATGA0384-50 µg, ATGA0384-100 µg, ATGA0384-250 µg, ATGA0384-500 µg, ATGA0384-1 mg, ATGA0384-10, ATGA0384-20, ATGA0384-50, ATGA0384-100, ATGA0384-250, ATGA0384-500, ATGA0384-1

References & Citations

Stoler A., et al. (1985) Proc Natl Acad Sci USA. 82 (2) : 570-574.; ; Choudhary C., et al. (2009) Science. 325: 834-840.; ; Bian Y., et al. (2014) J Proteomics. 96: 253-262.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_002952

Uniprot Accession Number

P26447

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : A549 cell lysate ; ; Lane 3. : NIH/3T3 cell lysate ; ; Lane 4. : 3T3-L1 cell lysate

IF Description

ICC/IF analysis of S100A4 in A549 cells. The cell was stained with ATGA0384 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of S100A4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining, Wheat germ agglutinin (WGA) with fluorescein conjugate (Red) for plasma membrane staining and monoclonal anti-human S100A4 antibody{2004010323173395.jpg}