Human COX5A antibody

COX5A, also known as COX, COX-VA and VA, belongs to the cytochrome c oxidase subunit 5A family. Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. COX5A is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Product Specifications

Product Name Alternative

Cytochrome c oxidase subunit Va, cytochrome c oxidase subunit Va, COX, COX-VA, VA

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human COX5A (42-150aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT7A6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

COX5A, cytochrome c oxidase subunit Va, cytochrome c oxidase subunit Va, COX, COX-VA, VA, ATGA0382-10 µg, ATGA0382-20 µg, ATGA0382-50 µg, ATGA0382-100 µg, ATGA0382-250 µg, ATGA0382-500 µg, ATGA0382-1 mg, ATGA0382-10, ATGA0382-20, ATGA0382-50, ATGA0382-100, ATGA0382-250, ATGA0382-500, ATGA0382-1

References & Citations

Lenka N., et al. (1998) Prog Nucleic Acid Res Mol Biol. 61: 309-344. ; ; Schmidt TR., et al. (2002) Gene. 286: 13-19.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_004246

Uniprot Accession Number

P20674

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COX5A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : A431 cell lysate ; ; Lane 2. : HeLa cell lysate

IF Description

ICC/IF analysis of COX5A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human COX5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .