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Human PGK1 antibody

Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP.

Product Specifications

Product Name Alternative

Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGKA, MIG10

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PGK1 (1-417aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2F4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PGK1, Phosphoglycerate kinase 1, Pgk-1, ATGA0381-10 µg, ATGA0381-20 µg, ATGA0381-50 µg, ATGA0381-100 µg, ATGA0381-250 µg, ATGA0381-500 µg, ATGA0381-1 mg, ATGA0381-10, ATGA0381-20, ATGA0381-50, ATGA0381-100, ATGA0381-250, ATGA0381-500, ATGA0381-1

References & Citations

Svaasand EK., et al. (2007) Muscle Nerve. 36 (5) : 679-684. ; ; Valentin C., et al. (1998) Hum Mutat. 12 (4) : 280-287.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000282

Uniprot Accession Number

P00558

WB Description

Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGK1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : LNCaP cell lysate ; ; Lane 3. : A549 cell lysate ; ; Lane 4. : HepG2 cell lysate ; ; Lane 5. : 293T cell lysate ; ; Lane 6. : K562 cell lysate ; ; Lane 7. : Jurkat cell lysate

IF Description

ICC/IF analysis of PGK1 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .
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