Human Otubain-1/OTUB1 antibody

Otubain 1, also known as OTUB1, is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases and inhibits cytokine gene transcription in the immune system via its interaction with an ubiquitin protease and E3 ubiquitin ligase. This protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function of polyubiquitin chain growth.

Product Specifications

Product Name Alternative

Ubiquitin thioesterase Otubain 1, OTB1, OTu1, ubiquitin thioesterase Otubain 1 Deubiquitinating enzyme OTuB1, OTu domain containing ubiquitin aldehyde binding protein 1, Otubain 1, ubiquitin specific processing protease OTuB1, ubiquitin thioesterase OTuB1.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human OTUB1 (1-271aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT17E10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Otubain1, OTUB1, ubiquitin thioesterase Otubain 1, OTB1, OTu1, ubiquitin thioesterase Otubain 1 Deubiquitinating enzyme OTuB1, OTu domain containing ubiquitin aldehyde binding protein 1, Otubain 1, ubiquitin specific processing protease OTuB1, ubiquitin thioesterase OTuB1., ATGA0380-10 µg, ATGA0380-20 µg, ATGA0380-50 µg, ATGA0380-100 µg, ATGA0380-250 µg, ATGA0380-500 µg, ATGA0380-1 mg, ATGA0380-10, ATGA0380-20, ATGA0380-50, ATGA0380-100, ATGA0380-250, ATGA0380-500, ATGA0380-1

References & Citations

Balakirev MY., et al. (2003) EMBO Rep. 4 (5) : 5175-5122.; ; Soares L., et al. (2004) Nat Immunol. 5 (1) : 45-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_060140

Uniprot Accession Number

Q96FW1

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OTUB1 antibody (1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : A549 cell lysate ; ; Lane 2. : HeLa cell lysate ; ; Lane 3. : HepG2 cell lysate ; ; Lane 4. : 293T cell lysate

IF Description

ICC/IF analysis of OTUB1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OTUB1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .