Human Peroxiredoxin 5/PRDX5 antibody

Peroxiredoxin 5, also known as PRDX5, is a member of the peroxiredoxin family of antioxidant enzymes, which reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. This protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. It has been reported that peroxiredoxin 5 is involved in intracellular redox signaling.

Product Specifications

Product Name Alternative

Alu corepressor 1, Antioxidant enzyme B166, AOEB166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Prx-V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, ACR1, AOEB166, PRXV, SBBI10

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Peroxiredoxin 5 (53-214aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT6A10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Peroxiredoxin5, Peroxiredoxin 5, ACR1, AOEB116, B116, PLP, PMP20, PRDX5, PRXV, SBBI10, AOEB166, PRDX 5, Thioredoxin reductase., ATGA0379-10 µg, ATGA0379-20 µg, ATGA0379-50 µg, ATGA0379-100 µg, ATGA0379-250 µg, ATGA0379-500 µg, ATGA0379-1 mg, ATGA0379-10, ATGA0379-20, ATGA0379-50, ATGA0379-100, ATGA0379-250, ATGA0379-500, ATGA0379-1

References & Citations

Yamashita H., et al. (1999) J Biol Chem. 274 (42) : 29897-29904. ; ; Declercg JP., et al. (2001) J Mol Biol. 311 (4) : 751-759.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Redox Proteins

NCBI Accession Number

NP_036226

Uniprot Accession Number

P30044

WB Description

The Recombinant Human PRDX5 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 5 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Recombinant PRDX5 protein ; ; Lane 2. : MCF-7 cell lysate ; ; Lane 3. : A549 cell lysate ; ; Lane 4. : HeLa cell lysate ; ; Lane 5. : 293T cell lysate ; ; Lane 6. : Jurkat cell lysate

FACS Description

Flow cytometry analysis of PRDX5 in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of PRDX5 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of PRDX5 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0379-IF2.jpg}