Human alpha-Actinin 1/ACTN1 antibody

ACTN1 also known as Actinin, alpha 1. ACTN1 functions as an actin-binding-protein. ACTN1 determines the motility of keratinocytes by regulating the organization of the actin cytoskeleton, focal adhesion, and hemidesmosome proteins complexes, thereby modulating cell speed, lamellipodial dynamics, and directed migration.

Product Specifications

Product Name Alternative

Alpha-actinin-1 isoform a, Actinin alpha 1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACTN1 (1-249aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1D10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Non-muscle alpha-actinin-1, F-actin cross-linking protein, Alpha-actinin-1 isoform a, Alpha-actinin cytoskeletal isoform, ACTN1, Actinin alpha 1, a-Actinin1, ATGA0377-10 µg, ATGA0377-20 µg, ATGA0377-50 µg, ATGA0377-100 µg, ATGA0377-250 µg, ATGA0377-500 µg, ATGA0377-1 mg, ATGA0377-010, ATGA0377-020, ATGA0377-050, ATGA0377-100, ATGA0377-250, ATGA0377-500, ATGA0377-01M

References & Citations

Hamill KJ., et al. (2014) Journal of Invest Dermatology. 135 (4) : 1043-1052. ; ; Kunishima S., et al. (2013) Am J Hum Genet. 92 (3) : 431-438.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001123476

Uniprot Accession Number

P12814

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTN1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : PC-3 cell lysate ; ; Lane 2. : A549 cell lysate

FACS Description

Flow cytometry analysis of ACTN1 in HeLa cells. The cell was stained with ATGA0377 at 2-5ug for 1x1^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)

IF Description

ICC/IF analysis of ACTN1 in Balb-3T3 cells. The cell was stained with ATGA0377 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .