Human Dihydrofolate Reductase/DHFR antibody

Dihydrofolate reductase (DHFR) catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX) -resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

Product Specifications

Product Name Alternative

Dihydrofolate reductase, EC 1.5.1.3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human DHFR (1-187aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT5B2

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Dihydrofolate Reductase, DHFR, Dihydrofolate reductase, EC 1.5.1.3, ATGA0376-10 µg, ATGA0376-20 µg, ATGA0376-50 µg, ATGA0376-100 µg, ATGA0376-250 µg, ATGA0376-500 µg, ATGA0376-1 mg, ATGA0376-10, ATGA0376-20, ATGA0376-50, ATGA0376-100, ATGA0376-250, ATGA0376-500, ATGA0376-1

References & Citations

Chen MJ., et al. (1984) J Biol Chem. 259 (6) : 3933-3943. ; ; Cody V., et al. (2009) Biochemistry. 48 (8) : 1702-1711.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Other

NCBI Accession Number

NP_000782

Uniprot Accession Number

P00374

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : Jurkat cell lysate ; ; Lane 3 : 293T cell lysate

IF Description

ICC/IF analysis of DHFR in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DHFR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .