Human NUDT1/MTH1 antibody

NUDT1, also known as MTH1 is a 179 amino acid cytoplasmic protein that is a member of the nudix hydrolase family. Highly expressed in testis, thymus and proliferating blood lymphocytes, NUDT1 functions as an antimutagenic that hydrolyzes oxidized purine nucleoside triphosphates to their corresponding monophosphates. Through its ability to enzymatically hydrolyze ATP and GTP to AMP and GMP, respectively, NUDT1 prevents misincorporation of GTP into DNA, thus preventing A:T to C:G transversions.

Product Specifications

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NUDT1 (1-156aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT3B3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

"7,8-dihydro-8-oxoguanine triphosphatase", 78-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21, MTH1, Nudix motif 1, NUDT1, ATGA0375-10 µg, ATGA0375-20 µg, ATGA0375-50 µg, ATGA0375-100 µg, ATGA0375-250 µg, ATGA0375-500 µg, ATGA0375-1 mg, ATGA0375-010, ATGA0375-020, ATGA0375-050, ATGA0375-100, ATGA0375-250, ATGA0375-500, ATGA0375-01M

References & Citations

Satoh J., et al. (2000) Eur J Neurol. 7 (6) : 673-677. ; ; Choi KM., et al. (2015) FEBS Lett. 589 (3) : 349-357.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_945191

Uniprot Accession Number

P36639

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NUDT1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: SW480 cell lysate; ; Lane 3.: THP-1 cell lysate; ; Lane 4.: Jurkat cell lysate; ; Lane 5.: A549 cell lysate

FACS Description

Flow cytometry analysis of NUDT1 in Jurkat cells. The cell was stained with ATGA0375 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of NUDT1 in A549 cells. The cell was stained with ATGA0375 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .