Human Glutathione S-transferase theta 1/GSTT1 antibody

Glutathione S-transferase theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes (alpha, mu, pi, theta, and zeta) . The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

Product Specifications

Product Name Alternative

Glutathione S-transferase theta 1, GSTT1, GST class-theta-1, Glutathione transferase T1-1, Glutathione S-transferase theta 1 EC 2.5.1.18, Glutathione S transferase 5, Glutathione S transferase theta 1, GST class theta 1, Glutathione transferase T1 1, GST 5 5, GST CL1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GSTT1 (1-240aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT38D11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GSTT1, ATGA0371-10 µg, ATGA0371-20 µg, ATGA0371-50 µg, ATGA0371-100 µg, ATGA0371-250 µg, ATGA0371-500 µg, ATGA0371-1 mg, ATGA0371-10, ATGA0371-20, ATGA0371-50, ATGA0371-100, ATGA0371-250, ATGA0371-500, ATGA0371-1

References & Citations

Webb G., et al. (1996) Genomics. 33 (1) : 121-123.; ; Mainwaring GW., et al. (1996) Biochem J. 318: 297-303.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Tags & Cell Markers

NCBI Accession Number

AAH07065

Uniprot Accession Number

P30711

WB Description

The recombinant protein (50ng), cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human GSTT1 protein; ; Lane 2.: HeLa cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: Jurkat cell lysate; ; Lane 5.: HL-60 cell lysate;

FACS Description

Flow cytometry analysis of GSTT1 in Hep3B cells. The cell was stained with ATGA0371 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of GSTT1 in HepG2 cells. The cell was stained with ATGA0371 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .