Human Glycine N-methyltransferase/GNMT antibody

Ras-related protein Glycine N-methyltransferase (GNMT) catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy) . Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Product Specifications

Product Name Alternative

Glycine N-methyltransferase, GNMT, Glycine N-methyltransferase, Glycine N-methyltransferase EC 2.1.1.20, Glycine N methyltransferase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GNMT (1-295aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT5D1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Glycine N-methyltransferase, GNMT, ATGA0370-10 µg, ATGA0370-20 µg, ATGA0370-50 µg, ATGA0370-100 µg, ATGA0370-250 µg, ATGA0370-500 µg, ATGA0370-1 mg, ATGA0370-10, ATGA0370-20, ATGA0370-50, ATGA0370-100, ATGA0370-250, ATGA0370-500, ATGA0370-1

References & Citations

Velichkova., et al. (2006) J Phys Chem B. 109: 8216-8219.; ; Huang YC., et al. (2008) J Gastroenterol Hepatol. 23: 1384.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_061833

Uniprot Accession Number

Q14749

WB Description

The recombinant protein (50ng) and tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human GNMT protein; ; Lane 2.: Mouse Liver tissue lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: GNMT Transfected 293T cell lysate {ATGA0370-WB2.jpg}

IF Description

ICC/IF analysis of GNMT in HepG2 cells. The cell was stained with ATGA0370 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .