Human KRT5 antibody

Keratin 5, also known as KRT5, K5, or CK5, dimerizes with keratin 14 and forms the intermediate filaments (IF) that make up the cytoskeleton of basal epithelial cells. This protein is involved in several diseases including epidermolysis bullosa simplex and breast and lung cancers. Keratin 5 (and K14) are expressed primarily in basal keratinocytes in the epidermis, specifically in the stratified epithelium lining the skin and digestive tract. Keratin intermediate filaments make up the cytoskeletal scaffold within epithelial cells, which contributes to the cell architecture and provides the cells with the ability to withstand mechanical, and non-mechanical, stresses. K5/K14 keratin pairs are able to undergo extensive bundling due to the non-helical tail of K15 acting as a weak cross-linker at the intermediate filament surface. This bundling increase the elasticity, and therefore the mechanical resilience, of the intermediate filaments.

Product Specifications

Product Name Alternative

Keratin 5, CK5, DDD, K5, KRT5A

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KRT5 (1-590aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT12C4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KRT5, ATGA0368-10 µg, ATGA0368-20 µg, ATGA0368-50 µg, ATGA0368-100 µg, ATGA0368-250 µg, ATGA0368-500 µg, ATGA0368-1 mg, ATGA0368-10, ATGA0368-20, ATGA0368-50, ATGA0368-100, ATGA0368-250, ATGA0368-500, ATGA0368-1

References & Citations

Lersch R., et al. (1988) Molecular and Cellular Biology. 8 (1) : 486-493.; ; Eckert RL., et al. (1988) Dna. 7 (5) : 337-345.; ; Atkinson SD., et al. (2011) J Invest Dermatol. 131 (10) : 2079-2086.; ; van de Rijn M., et al. (2002) Am J Pathol. 161 (6) : 1991-1996.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000415

Uniprot Accession Number

P13647

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate; ; Lane 2. : A431 cell lysate

IHC description

Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT5 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

FACS Description

Flow cytometry analysis of KRT5 in HeLa cells. The cell was stained with ATGA0368 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of KRT5 in A549 cells. The cell was stained with ATGA0368 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .