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Human CAPNS1 antibody

CAPNS1, also known as Calpain small subunit 1, are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Two transcript variants encoding the same protein have been identified for this gene.

Product Specifications

Product Name Alternative

Calpain small subunit 1, Calpain, small subunit 1, 30K, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CAPNS1 (84-268aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1D11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CAPNS1, ATGA0367-10 µg, ATGA0367-20 µg, ATGA0367-50 µg, ATGA0367-100 µg, ATGA0367-250 µg, ATGA0367-500 µg, ATGA0367-1 mg, ATGA0367-10, ATGA0367-20, ATGA0367-50, ATGA0367-100, ATGA0367-250, ATGA0367-500, ATGA0367-1

References & Citations

Miyake S., et al. (1987) Nucleic Acids Res. 14 (22) : 8805-8817.; ; Ohno S., et al. (1986) Nucleic Acids Res. 14 (13) : 5559.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_001740

Uniprot Accession Number

P04632

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPNS1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: A431 cell lysate; ; Lane 3.: 293T cell lysate;

IF Description

ICC/IF analysis of CAPNS1 in HeLa cells. The cell was stained with ATGA0367 (1:100) . The secondary antibody (green) was used Alexa Fluor 488.

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