Human Lactate Dehydrogenase B/LDHB antibody

LDHB, also known as L-lactate dehydrogenase B chain, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD) . The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors.

Product Specifications

Product Name Alternative

Renal carcinoma antigen NY-REN-46, Lactate dehydrogenase B, L-lactate dehydrogenase B chain, LDH-H, LDH-B, LDH heart subunit

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human LDHB (1-334aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT14D7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Lactate Dehydrogenase B, LDHB, ATGA0365-10 µg, ATGA0365-20 µg, ATGA0365-50 µg, ATGA0365-100 µg, ATGA0365-250 µg, ATGA0365-500 µg, ATGA0365-1 mg, ATGA0365-10, ATGA0365-20, ATGA0365-50, ATGA0365-100, ATGA0365-250, ATGA0365-500, ATGA0365-1

References & Citations

Read JA., et al. (2001) Proteins. 43 (2) : 175-185. ; ; Butt AA., et al. (2002) AIDS. 12 (7) : 317-321.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_002291

Uniprot Accession Number

P07195

WB Description

The Recombinant Human LDHB (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Recombinant LDHB protein; ; Lane 2. : A549 cell lysate; ; Lane 3. : 293T cell lysate; ; Lane 4. : Jurkat cell lysate

IF Description

ICC/IF analysis of LDHB in HeLa cells. The cell was stained with ATGA0365 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .