Human Carbonyl reductase 1/CBR1 antibody

Carbonyl reductase 1 (CBR1) is a NADPH-dependent, monomeric, and cytosolic enzyme belonging to a family of short-chain dehydrogenases/reductases. This protein consists of 277 amino acid residues and is widely distributed in human tissues such as liver, epidermis, stomach, small intestine, kidney, neuronal cells, and smooth muscle fiber. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer drug, doxorubicin. The best substrates of CBR1 are quinones, including ubiquinone-1 and tocophrolquinone (vitamin E) .

Product Specifications

Product Name Alternative

Carbonyl reductase 1, Carbonyl reductase 1, CBR, hCBR1, SDR21C1, CBR1, Carbonyl reductase 1 15 hydroxyprostaglandin dehydrogenase [NADP+], Carbonyl reductase [NADPH] 1.CBR 1, CRN, NADPH dependent carbonyl reductase 1, Prostaglandin 9 ketoreductase, Prostaglandin E (2) 9 reductase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CBR1 (1-277aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2D6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CBR1, ATGA0364-10 µg, ATGA0364-20 µg, ATGA0364-50 µg, ATGA0364-100 µg, ATGA0364-250 µg, ATGA0364-500 µg, ATGA0364-1 mg, ATGA0364-10, ATGA0364-20, ATGA0364-50, ATGA0364-100, ATGA0364-250, ATGA0364-500, ATGA0364-1

References & Citations

Lemieux N., et al. (1993) Genomics. 15 (1) : 169-172.; ; Wermuth B., et al. (1986) . Biochem Pharmacol. 35 (8) : 1277-1282.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_001748

Uniprot Accession Number

P16152

WB Description

The Recombinant Human CBR1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human CBR1; ; Lane 2. : HeLa cell lysate; ; Lane 3. : 293T cell lysate; ; Lane 4. : MCF-7 cell lysate; ; Lane 5. : HepG2 cell lysate

FACS Description

Flow cytometry analysis of CBR1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of CBR1 in HeLa cells. The cell was stained with ATGA0364 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .