What is the catalog number for Human Cyclin H antibody?

The catalog number for Human Cyclin H antibody is ATGA0363-050.

What sizes are available for Human Cyclin H antibody?

Human Cyclin H antibody is available in the following size(s): 50 µL.

Is Human Cyclin H antibody in stock?

Human Cyclin H antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Human Cyclin H antibody

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK) . This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

Product Specifications

Product Name Alternative

CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Cyclin H (1-323aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT3G6

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cyclin H, CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H, ATGA0363-10 µg, ATGA0363-20 µg, ATGA0363-50 µg, ATGA0363-100 µg, ATGA0363-250 µg, ATGA0363-500 µg, ATGA0363-1 mg, ATGA0363-10, ATGA0363-20, ATGA0363-50, ATGA0363-100, ATGA0363-250, ATGA0363-500, ATGA0363-1

References & Citations

Jeang KT., et al. (1998) J Biomed Sci. 5 (1) : 24-27.; ; Yankulov K., et al. (1998) Current Biology. 8 (13) : 447-449.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_001230

Uniprot Accession Number

P51946

WB Description

The recombinant proteins (25ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human Cyclin H. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate ; ; Lane 2.: Jurkat cell lysate ; ; Lane 3.: Ramos cell lysate ; ; Lane 4.: Balb-3T3 cell lysate {ATGA0363-WB2.jpg}