Human Peroxiredoxin 6/PRDX6 antibody

Peroxiredoxin 6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell. it can reduce H (2) O (2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Product Specifications

Product Name Alternative

1-Cys peroxiredoxin, 1-Cys PRX, 24 kDa protein, Acidic calcium-independent phospholipase A2, AiPLA2, Antioxidant protein 2, Glutathione-dependent peroxiredoxin Liver 2D page spot 40, Lysophosphatidylcholine acyltransferase 5, Non-selenium glutathione peroxidase, NSGPx, Red blood cells page spot 12, AOP2, KIAA0106, LPCAT-5

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PRDX6 (1-224aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT22E7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Peroxiredoxin6, ATGA0362-10 µg, ATGA0362-20 µg, ATGA0362-50 µg, ATGA0362-100 µg, ATGA0362-250 µg, ATGA0362-500 µg, ATGA0362-1 mg, ATGA0362-10, ATGA0362-20, ATGA0362-50, ATGA0362-100, ATGA0362-250, ATGA0362-500, ATGA0362-1

References & Citations

Phelan SA., et al. (2001) Antioxid Redox Signal. 1 (4) : 571-584.; ; Trevisan R., et al. (2014) Comp Biochem Physiol C Toxicol Phamacol. 159: 22-30.; ; McDonagh B., et al. (2014) J Proteome Res. 13: 5008-5021.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Redox Proteins

NCBI Accession Number

NP_004896

Uniprot Accession Number

P30041

WB Description

The recombinant human PRDX6 protein (50ng), Cell and Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 6 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant PRDX6 protein; ; Lane 2.: HeLa cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: HepG2 cell lysate; ; Lane 5.: U87MG cell lysate; ; Lane 6.: Ramos cell lysate

FACS Description

Flow cytometry analysis of Peroxiredoxin in HeLa cells. The cell was stained with ATGA0362 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of PRDX6 in HeLa cells. The cell was stained with ATGA0362 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .