Human Fascin/FSCN1 antibody

FSCN1 is an actin-bundling protein that provides rigidity to filopodial bundles to efficiently push the membrane forward during cytoskeleton remodeling and cell migration. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. And this protein is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. FSCN1 expression has been localized to neurons, glial cells, and endothelial cells.

Product Specifications

Product Name Alternative

Fascin, FAN1, HSN, SNL, Fascin

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FSCN1 (1-493aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT13D2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Fascin, FSCN1, ATGA0361-10 µg, ATGA0361-20 µg, ATGA0361-50 µg, ATGA0361-100 µg, ATGA0361-250 µg, ATGA0361-500 µg, ATGA0361-1 mg, ATGA0361-10, ATGA0361-20, ATGA0361-50, ATGA0361-100, ATGA0361-250, ATGA0361-500, ATGA0361-1

References & Citations

Wu D., et al. (2010) Ann Oncol. 21 (3) : 589-596. ; ; Shin H., et al. (2009) Phys Rev Left. 103 (23) : 238102.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003079

Uniprot Accession Number

Q16658

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FSCN1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : THP-1 cell lysate

FACS Description

Flow cytometry analysis of FSCN1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of FSCN1 in HeLa cells. The cell was stained with ATGA0361 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .