Human PA28 alpha/PSME1 antibody

PSME1, also known as PA28 alpha, is an interferon gamma (IFNG) inducible proteasome activator required for presentation of certain major histocompatibility (MHC) class I antigens. The PSME1 complex is an alternative proteasome activator that does not employ the use of ubiquitin. The PSME1 complex is composed of two homologous subunits, alpha and beta, which have similar catalytic properties and associate to form a hexameric ring.

Product Specifications

Product Name Alternative

Proteasome activator subunit 1, 11S regulator complex subunit alpha, REG-alpha, Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, Proteasome activator 28 subunit alpha, PA28a, PA28alpha, IFI5111

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PSME1 (1-249aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT12H3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

REG-alpha, REGalpha, PSME1, Proteasome activator subunit 1, Proteasome activator complex subunit 1, Proteasome activator 28 subunit alpha, PA28alpha, PA28a, PA28 Activator a Subunit, MGC8628, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111, Activator of multicatalytic protease subunit 1, 11S regulator complex subunit alpha, REG alpha, PA28 alpha, PA28 A, Interferon gamma up regulated I-5111 protein, Interferon gamma up regulated I5111 protein, ATGA0360-10 µg, ATGA0360-20 µg, ATGA0360-50 µg, ATGA0360-100 µg, ATGA0360-250 µg, ATGA0360-500 µg, ATGA0360-1 mg, ATGA0360-010, ATGA0360-020, ATGA0360-050, ATGA0360-100, ATGA0360-250, ATGA0360-500, ATGA0360-01M

References & Citations

Ahn K., et al. (1996) J Biol Chem. 271 (30) : 18237-18242. ; ; Murata S., et al. (2001) EMBO J. 20 (21) : 5898-5907.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Ubiquitination

NCBI Accession Number

NP_006254

Uniprot Accession Number

Q06323

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSME1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : LNCap cell lysate ; ; Lane 3. : A549 cell lysate ; ; Lane 4. : 293T cell lysate ; ; Lane 5. : Jurkat cell lysate ; ; Lane 6. : Raji cell lysate ; ; Lane 7. : A431 cell lysate

IF Description

ICC/IF analysis of PSME1 in PC3 cells. The cell was stained with ATGA0360 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .