Human ACOT8 antibody

Acyl-coenzyme A thioesterase 8 is an enzyme that in humans is encoded by the ACOT8 gene. The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene.

Product Specifications

Product Name Alternative

Acyl-coenzyme A thioesterase 8, hACTE-III, HNAACTE, the, PTE-1, PTE-2, PTE1, PTE2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACOT8 (1-319aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT4D10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ACOT8, ATGA0353-10 µg, ATGA0353-20 µg, ATGA0353-50 µg, ATGA0353-100 µg, ATGA0353-250 µg, ATGA0353-500 µg, ATGA0353-1 mg, ATGA0353-10, ATGA0353-20, ATGA0353-50, ATGA0353-100, ATGA0353-250, ATGA0353-500, ATGA0353-1

References & Citations

Jones JM., et al. (1999) J Biol Chem. 274 (14) : 9216-9223.; ; Liu LX., et al. (1997) J Biol Chem. 272 (21) : 13779-13785.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_005460

Uniprot Accession Number

O14734

WB Description

The recombinant Human ACOT8 (10ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT8 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human ACOT8; ; Lane 2.: Ramos cell lysate; ; Lane 3.: A431 cell lysate; ; Lane 4 : 293T cell lysate; ; Lane 5 : A549 cell lysate

FACS Description

Flow cytometry analysis of ACOT8 in CTLL-2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of ACOT8 in A549 cells. The cell was stained with ATGA0353 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .