Human Ras antibody

All RAS protein family members belong to a class of protein called small GTPase, and are involved cellular signal transduction. RAS is 'switched on' by incoming signals, it subsequently switches on other proteins, which ultimately turn on genes involved in cell growth, differentiation and survival. Mutations in RAS genes can lead to the production of permanently activated RAS proteins. This can cause unintended and overactive signaling inside the cell, even in the absence of incoming signals. Because these signals result in cell growth and division, overactive RAS signaling can ultimately lead to cancer. The 3 Ras genes in humans (HRas, KRas, NRas) are the most common oncogenes in human cancer; mutations that permanently activate RAS are found in 20% to 25% of all human tumors and up to 90% in certain types of cancer. RAS inhibitors are being studied as a treatment for cancer, and other diseases with RAS overexpression.

Product Specifications

Product Name Alternative

GTPase Kras, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, NS3, RASK2, GTPase Kras, K-RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS 1, NS 3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KRAS (1-186aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2F8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Ras, ATGA0350-10 µg, ATGA0350-20 µg, ATGA0350-50 µg, ATGA0350-100 µg, ATGA0350-250 µg, ATGA0350-500 µg, ATGA0350-1 mg, ATGA0350-10, ATGA0350-20, ATGA0350-50, ATGA0350-100, ATGA0350-250, ATGA0350-500, ATGA0350-1

References & Citations

McGrath JP., et al. (1983) Nature. 304 (5926) : 501-506.; ; Schubbert S., et al. (2006) Nat Genet. 38 (3) : 331-336.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_203524

Uniprot Accession Number

P01116

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : Ramos cell lysate; ; Lane 4. : A549 cell lysate; ; Lane 5. : Balb/3T3 cell lysate The Recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : KRAS recombinant protein (ATGP2062) ; ; Lane 2. : HRAS recombinant protein (ATGP0516) ; ; Lane 3. : NRAS recombinant protein (ATGP0492) {ATGA0350-WB2.jpg}

IF Description

ICC/IF analysis of RAS in HeLa cells. The cell was stained with ATGA0350 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .