Human PMVK antibody

Phosphomevalonate kinase, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.

Product Specifications

Product Name Alternative

Phosphomevalonate kinase, HuMPMKI, PMK, PMKA, PMKASE, Phosphomevalonate kinase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PMVK (1-192aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT21C10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PMVK, ATGA0349-10 µg, ATGA0349-20 µg, ATGA0349-50 µg, ATGA0349-100 µg, ATGA0349-250 µg, ATGA0349-500 µg, ATGA0349-1 mg, ATGA0349-10, ATGA0349-20, ATGA0349-50, ATGA0349-100, ATGA0349-250, ATGA0349-500, ATGA0349-1

References & Citations

Cho YK., et al. (2001) J Biol chem. 278: 4510-4515.; ; Hogenboom S., et al. (2004) J Lipid Res. 45: 697-705.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_006547

Uniprot Accession Number

Q15126

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : Jukat cell lysate; ; Lane 4. : MCF7 cell lysate; ; Lane 5. : A549 cell lysate

FACS Description

Flow cytometry analysis of PMVK in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of PMVK in PC3 cells. The cell was stained with ATGA0349 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .