Human GRP94/HSP90B1 antibody

GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.

Product Specifications

Product Name Alternative

Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GRP94 (22-803aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT94B9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GRP9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein, 94 kDa glucose regulated protein, 94kDa glucose regulated protein, 94kDa glucose-regulated protein, ATGA0348-10 µg, ATGA0348-20 µg, ATGA0348-50 µg, ATGA0348-100 µg, ATGA0348-250 µg, ATGA0348-500 µg, ATGA0348-1 mg, ATGA0348-010, ATGA0348-020, ATGA0348-050, ATGA0348-100, ATGA0348-250, ATGA0348-500, ATGA0348-01M

References & Citations

Randow F., et al. (2001) Nat Cell Biol. 3 (10) : 231.; ; Li Z., et al. (2002) Front Biosci. 7: 731-751.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_003290

Uniprot Accession Number

P14625

WB Description

The Recombinant protein (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human GRP94 ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: Jurkat cell lysate ; ; Lane 4.: HepG2 cell lysate

FACS Description

Flow cytometry analysis of GRP94 in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of GRP94 in U87MG cells. The cell was stained with ATGA0348 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .