Human Glypican 4/GPC4 antibody

The Glypican (GPC) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of Glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the initial stage of neural tube closure, suggesting that GPC4 is unlikely to play an essential role in convergent extension in the mouse.

Product Specifications

Product Name Alternative

Glypican 4, Glypican 4, dJ900E8.1, glypican proteoglycan 4

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GPC4 (401-529aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT51E3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Glypican4, GPC4, ATGA0346-10 µg, ATGA0346-20 µg, ATGA0346-50 µg, ATGA0346-100 µg, ATGA0346-250 µg, ATGA0346-500 µg, ATGA0346-1 mg, ATGA0346-10, ATGA0346-20, ATGA0346-50, ATGA0346-100, ATGA0346-250, ATGA0346-500, ATGA0346-1

References & Citations

Ybot-Gonzalez P., et al. (2005) Dev Dyn. 233 (3) : 1013-1017.; ; Karihaloo A., et al. (2004) Mol Cell Biol. 24 (19) : 8745-8752.; ; Ohkawara B., et al. (2003) Development. 130 (10) : 2129-2138.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001439

Uniprot Accession Number

O75487

WB Description

The Recombinant portein (50ng) and Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPC4 anibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Recombinant Human GPC4 ; ; Lane 2. : Ramos cell lysate

FACS Description

Flow cytometry analysis of GPC4 in 293T cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of GPC4 in 293T cells. The cell was stained with ATGA0346 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .