Human beta-III Tubulin antibody

Tubulin Beta 3 is 450 amino acid protein. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Its expression is primarily restricted to central and peripheral nervous system.

Product Specifications

Product Name Alternative

Tubulin beta 3 class III, Tubulin, beta 3 class III, beta-4, CDCBM, CFEOM3A, TuBB4, TUBB3, FEOM3, fibrosis of extraocular muscles, congenital

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TUBB3 (1-450aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2F5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

B-3 Tubulin, ATGA0343-10 µg, ATGA0343-20 µg, ATGA0343-50 µg, ATGA0343-100 µg, ATGA0343-250 µg, ATGA0343-500 µg, ATGA0343-1 mg, ATGA0343-10, ATGA0343-20, ATGA0343-50, ATGA0343-100, ATGA0343-250, ATGA0343-500, ATGA0343-1

References & Citations

Qu C., et al. (2013) J Cell Sci. 126 (14) : 3070-3081.; ; Marczak A., et al. (2015) Postepy Hig Med Dosw. 69: 158-164.; ; McCarroll J.A., et al. (2015) Cancer Res. 75 (2) : 415-425.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_006077

Uniprot Accession Number

Q13509

WB Description

The cell lysates (40ug) and tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tubulin Beta 3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: 293T cell lysate ; ; Lane 3.: Mouse Brain tissue lysate

FACS Description

Flow cytometry analysis of Tubulin Beta 3 in U-87MG cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of Tubulin Beta 3 in U-87MG cells. The cell was stained with ATGA0343 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .