Human Cytokeratin 17/KRT17 antibody

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin 17 is involved in wound healing and cell growth, two processes that require rapid cytoskeletal remodeling. Keratinocytes deficient in keratin 17 exhibit abnormal Akt/mTOR signaling and fail to produce an increase in translation, cell size, or growth; these cells also exhibit abnormal 14-3-3 sigma localization.

Product Specifications

Product Name Alternative

Keratin type I cytoskeletal 17, K17, PC, PC2, PCHC1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KRT17 (1-432aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT9F3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

K17, Keratin, Keratin type I cytoskeletal 17, KRT17, PC, PC2, PCHC1, type I cytoskeletal 17, ATGA0340-10 µg, ATGA0340-20 µg, ATGA0340-50 µg, ATGA0340-100 µg, ATGA0340-250 µg, ATGA0340-500 µg, ATGA0340-1 mg, ATGA0340-010, ATGA0340-020, ATGA0340-050, ATGA0340-100, ATGA0340-250, ATGA0340-500, ATGA0340-01M

References & Citations

Kim S., et al. (2006) Nature. 441 (7091) : 362-365.; ; Troyanovsky S.M., et al. (1992) Eur J Cell Biol. 59 (1) : 127-137.; ; McGowan K.M. and Coulombe P.A. (1998) J Cell Biol. 143 (2) : 469-486.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_000413

Uniprot Accession Number

Q04695

WB Description

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT17 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human KRT17; ; Lane 2.: HeLa cell lysate; ; Lane 3.: A431 cell lysate

FACS Description

Flow cytometry analysis of KRT17 in A431 cells. The cell was stained with ATGA0340 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of KRT17 in HeLa cells. The cell was stained with ATGA0340 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of KRT17 in A431 cells. The cell was stained with ATGA0340 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0340-IF2.jpg}