Human Keratin 14/KRT14 antibody

Keratin 14 (KRT14) is a member of the type I keratin family of intermediate filament proteins. KRT14 gene provides instructions for making a protein called keratin 14. Keratins are a group of tough, fibrous proteins that form the structural framework of certain cells, particularly cells that make up the skin, hair, and nails. Keratin 14 is specifically produced in cells called keratinocytes in the outer layer of the skin.

Product Specifications

Product Name Alternative

Keratin 14, cytokeratin 14, GIG36, MGLAP, NTI

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KRT14 (1-472aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT13F4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cytokeratin14, ATGA0338-10 µg, ATGA0338-20 µg, ATGA0338-50 µg, ATGA0338-100 µg, ATGA0338-250 µg, ATGA0338-500 µg, ATGA0338-1 mg, ATGA0338-10, ATGA0338-20, ATGA0338-50, ATGA0338-100, ATGA0338-250, ATGA0338-500, ATGA0338-1

References & Citations

Pfendner E.G., et al. (2005) J Invest Dermatol. 125 (2) : 239-243.; ; Arin M.J., et al (2010) Br J Dermatol. 162 (6) : 1365-1369.; ; Muller F.B., et al (2006) Hum Mutat. 27 (7) : 719-720.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

AAH19097

Uniprot Accession Number

P02533

WB Description

The recombinant protein (50ng) and cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT14 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human KRT14 ; ; Lane 2.: A431 cell lysate

IHC description

Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT14 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .

FACS Description

Flow cytometry analysis of KRT14 in A431 cells. The cell was stained with ATGA0338 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of KRT14 in PC3 cells. The cell was stained with ATGA0338 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .