Human Cytokeratin 20/KRT20 antibody

Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to KRT20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the KRT20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumor.

Product Specifications

Product Name Alternative

CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KRT20 (1-424aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT10D8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KRT20, Keratin type I cytoskeletal 20, Keratin, type I cytoskeletal 20, CD20, CK-20, CK20, K20, KRT21, ATGA0337-10 µg, ATGA0337-20 µg, ATGA0337-50 µg, ATGA0337-100 µg, ATGA0337-250 µg, ATGA0337-500 µg, ATGA0337-1 mg, ATGA0337-010, ATGA0337-020, ATGA0337-050, ATGA0337-100, ATGA0337-250, ATGA0337-500, ATGA0337-01M

References & Citations

Moll R., et al. (1990) J Cell Biol. 111 (2) : 567-580.; ; Piero D., et al. (2011) Nat Biotechnol. 29 (12) : 1120-1127.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_061883

Uniprot Accession Number

P35900

WB Description

The Recombinant protein (25ng) and Cell lysates of HeLa, A431 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human KRT20 ; ; Lane 2.: A431 cell lysate

IF Description

ICC/IF analysis of KRT20 in A549 cells. The cell was stained with ATGA0337 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .