Human ACAT2 antibody

Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT (acetyl-Coenzyme A acetyltransferase 2) gene. ACAT2 is an acetyl-CoA C-acetyltransferase enzyme, plays a role in regulating lipid metabolism. It catalyzes the synthesis of acetoacetyl-CoA from two acetyl-CoA molecules, which is later converted into steroids.

Product Specifications

Product Name Alternative

Acetyl-CoA acetyltransferase cytosolic, ACTL, Acetyl-CoA acetyltransferase, cytosolic

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACAT2 (1-397aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT55D5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ACAT2, ATGA0335-10 µg, ATGA0335-20 µg, ATGA0335-50 µg, ATGA0335-100 µg, ATGA0335-250 µg, ATGA0335-500 µg, ATGA0335-1 mg, ATGA0335-10, ATGA0335-20, ATGA0335-50, ATGA0335-100, ATGA0335-250, ATGA0335-500, ATGA0335-1

References & Citations

Shintani S., et al. (1999) Genetics. 152 (2) : 743-754.; ; Middleton B., et al. (1974) Biochem J. 139 (1) : 109-121.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Actin & Myosin Related

NCBI Accession Number

NP_005882

Uniprot Accession Number

Q9BWD1

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human ACAT2 protein; ; The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: ACAT2 Transfected 293T cell lysate; ; {ATGA0335-WB2.jpg}The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: Ramos cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: HL-60 cell lysate; ; Lane 5.: SW480 cell lysate; ; {ATGA0335-WB3.jpg}

FACS Description

Flow cytometry analysis of ACAT2 in PC3 cells. The cell was stained with ATGA0335 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of ACAT2 in HeLa cells. The cell was stained with ATGA0335 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .