Human PDCL3 antibody

PDCL3 (phosducin-like 3), also known as VIAF1 (viral IAP-associated factor 1), VIAF, PHLP3 or HTPHLP, is a widely expressed protein that belongs to the phosducin-like family of proteins. phosducin-like family members associate with the cytosolic chaperonin complex (CCT) and regulate the folding of proteins. Localizing to the cytoplasm, PDCL3 does not appear to play a role in G protein signaling, but participates in the chaperone-assisted folding of proteins, such as beta Tubulin and Actin, that are involved in the regulation of cell cycle progression. More specifically, PDCL3, when associated with CCT, represses the ATPase activity of CCT and ultimately disrupts the folding of the Actin or tubulin substrates.

Product Specifications

Product Name Alternative

Phosducin-Like protein 3, Phosducin-Like protein 3, HTPHLP, MGC3062, PHLP2A, PHLP3, VIAF, VIAF1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PDCL3 (1-239aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT8F9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PDCL3, ATGA0333-10 µg, ATGA0333-20 µg, ATGA0333-50 µg, ATGA0333-100 µg, ATGA0333-250 µg, ATGA0333-500 µg, ATGA0333-1 mg, ATGA0333-10, ATGA0333-20, ATGA0333-50, ATGA0333-100, ATGA0333-250, ATGA0333-500, ATGA0333-1

References & Citations

Wilkinson J.C., et al. (2004) Biol. Chem. 279 (49) :51091-51099.; ; Hillier L.W., et al. (2005) Nature 434 (7034) :724-731.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_076970

Uniprot Accession Number

Q9H2J4

WB Description

The cell lysates of Jurkat (40ug), A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: A549 cell lysateThe Recombinant protein (100ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Protein PDCL3 100ng{ATGA0333-WB2.jpg}

IF Description

ICC/IF analysis of PDCL3 in HeLa cells. The cell was stained with ATGA0333 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of PDCL3 in PC3 cells. The cell was stained with ATGA0333 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0333-IF2.jpg}