Human BMP-8b antibody

Bone morphogenic proteins (BMPs) are members of the TGF beta superfamily. BMPs are involved in the induction of cartilage and bone formation. Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. BMP-8 is thought to be involved in early development, as detectable expression has not been found in adult organs. Two BMP-8 proteins exist, namely BMP-8A and BMP-8B (also designated OP-2), and are encoded by two distinct genes.

Product Specifications

Product Name Alternative

Bone morphogenetic protein 8B, BMP8, OP2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BMP8b (264-402aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT13E6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

BMP8b, ATGA0332-10 µg, ATGA0332-20 µg, ATGA0332-50 µg, ATGA0332-100 µg, ATGA0332-250 µg, ATGA0332-500 µg, ATGA0332-1 mg, ATGA0332-10, ATGA0332-20, ATGA0332-50, ATGA0332-100, ATGA0332-250, ATGA0332-500, ATGA0332-1

References & Citations

Ozkaynak E., et al. (1992) J Biol Chem. 267 (35) :25220-25227.; ; Gregory SG., et al. (2006) Nature. 441 (7091) :315-321.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_001711

Uniprot Accession Number

P34820

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: BMP8b transfected 293T cell lysate The Recombinant BMP8b proteins (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0332-WB2.jpg}

IF Description

ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with ATGA0332 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .