Human CD3 epsilon/CD3E antibody

The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multi subunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3) . It is composed of five invariant polypeptide chains that associate to form three dimers. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

Product Specifications

Product Name Alternative

T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CD3E (23-126aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT3H3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CD3 epsilon, CD3E, ATGA0331-10 µg, ATGA0331-20 µg, ATGA0331-50 µg, ATGA0331-100 µg, ATGA0331-250 µg, ATGA0331-500 µg, ATGA0331-1 mg, ATGA0331-10, ATGA0331-20, ATGA0331-50, ATGA0331-100, ATGA0331-250, ATGA0331-500, ATGA0331-1

References & Citations

Gold DP., et al. (1986) Nature 321 (6068) : 431-434.; ; Soudais C., et al. (1993) Nat Genet 3 (1) :77-81.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_000724

Uniprot Accession Number

P07766

WB Description

The cell lysate of Jurkat (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Recombinant CD3E proteins (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0331-WB2.jpg}

FACS Description

Flow cytometry analysis of CD3E in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of CD3E in Jurkat cells. The cell was stained with ATGA0331 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .