Human HABP1/C1QBP antibody

Complement component 1 Q subcomponent-binding protein, mitochondrial is a protein that in humans is encoded by the C1QBP gene. The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein.

Product Specifications

Product Name Alternative

Complement C1q binding protein, Complement component 1, Q subcomponent binding protein, GC1QBP, Glycoprotein gC1Q-R, gC1qR, Mitochondrial matrix protein p32, ASF/SF2-associated protein p32, SF2p32, C1q globular domain-binding protein, Hyaluronan-binding protein 1, Splicing factor SF2-associated protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human C1QBP (74-282aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1G7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HABP1, C1QBP, ATGA0330-10 µg, ATGA0330-20 µg, ATGA0330-50 µg, ATGA0330-100 µg, ATGA0330-250 µg, ATGA0330-500 µg, ATGA0330-1 mg, ATGA0330-10, ATGA0330-20, ATGA0330-50, ATGA0330-100, ATGA0330-250, ATGA0330-500, ATGA0330-1

References & Citations

Deb TB, et al. (1996) J Biol Chem 271 (4) :2206-12. ; ; Ghebrehiwet B, et al. (1994) J Exp Med 179 (6) :1809-21.; ; Entrez Gene: C1QBP complement component 1, q subcomponent binding protein.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001203

Uniprot Accession Number

Q07021

WB Description

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C1QBP antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Recombinant protein ; ; Lane 2 : HeLa cell lysate ; ; Lane 3 : Jurkat cell lysate ; ; Lane 4 : MCF7 cell lysate ; ; Lane 5 : Ramos cell lysate ; ; Lane 6 : 293T cell lysate ; ; Lane 7 : A549 cell lysate

IF Description

ICC/IF analysis of C1QBP in HeLa cells. The cell was stained with ATGA0330 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .