Human CRADD antibody

CRADD, also referred as RAIDD, is an adaptor protein that could interact with both caspase 2 and RIP that can promote apoptosis once activated. RAIDD (RIP-associated ICH-1/Ced-3 homologous protein with a death domain) has been identified as a RIP binding protein that also associates with members of the caspase family, providing a link between activation of the TNF-Rs and the triggering of the cysteine protease cascade. The amino-terminal domain of RAIDD shares significant homology with the prodomain of ICH-1 and mediates the binding of RAIDD to this cysteine protease.

Product Specifications

Product Name Alternative

Caspase and RIP adapter with death domain, RAIDD, CRADD, Caspase and RIP adapter with death domain, Caspase and RIP adapter with death domain CASP2 and RIPK1 domain containing adaptor with death domain, Death adaptor molecule RAIDD, Death domain containing protein CRADD, MGC9163, RIP associated ICH1/CED3 homologous protein with death domain, RIP associated protein with a death domain.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CRADD (1-199aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1a κ

Clone

AT14G8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CRADD, ATGA0327-10 µg, ATGA0327-20 µg, ATGA0327-50 µg, ATGA0327-100 µg, ATGA0327-250 µg, ATGA0327-500 µg, ATGA0327-1 mg, ATGA0327-10, ATGA0327-20, ATGA0327-50, ATGA0327-100, ATGA0327-250, ATGA0327-500, ATGA0327-1

References & Citations

Lin. Q., et al. (2012) J Immunol 188 (6) : 2493-2497.; ; Shen. Q., et al. (2014) Am J Transl Res 6 (5) : 538-547.; ; Ahmad. M., et al. (1997) Cancer Res 57 (4) : 615-619.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_003796

Uniprot Accession Number

P78560

WB Description

The cell lysates of A549, HeLa, MCF-7 and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: A549 cell lysate ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: MCF-7 cell lysate ; ; Lane 4.: 293T cell lysateThe Recombinant Human CRADD (50,100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Protein 50 ng ; ; Lane 2.: Recombinant Protein 100 ng{ATGA0327-WB2.jpg}

IF Description

ICC/IF analysis of CRADD in HeLa cells. The cell was stained with ATGA0327 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of CRADD in PC3 cells. The cell was stained with ATGA0327 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0327-IF2.jpg}