Human CD64/FCGR1A antibody

High affinity immunoglobulin gamma Fc receptor I (FCGR1A) is a protein that in humans is encoded by the FCGR1A gene. Also, This protein is known as CD64, belongs to the immunoglobulin superfamily. It is a high-affinity Fc-gamma receptor. They are playing a role in both innate and adaptive immune responses. They have been shown to interact with FCAR. The FcgRI binds the Fc portion of IgG and causes activation of the host cell via an intercellular ITAM motif.

Product Specifications

Product Name Alternative

High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, RIA, FcgammaRIa, fc-gamma RIA, CD64, FCGR1A, FCG1, FCGR1, IGFR1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FCGR1A (16-292aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT37F7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Fc g R1, CD64, High affinity immunoglobulin gamma Fc receptor I, CD64, CD64A, FCRI, IGFR1, ATGA0324-10 µg, ATGA0324-20 µg, ATGA0324-50 µg, ATGA0324-100 µg, ATGA0324-250 µg, ATGA0324-500 µg, ATGA0324-1 mg, ATGA0324-10, ATGA0324-20, ATGA0324-50, ATGA0324-100, ATGA0324-250, ATGA0324-500, ATGA0324-1, Fc gamma RIa

References & Citations

Maresco. D.L., et al. (1996) Cytogenet Cell Genet 73 (3) : 157-163.; ; Maresco. D.L., et al. (1998) Cytogenet Cell Genet 82 (1-2) : 71-74.; ; Morton., et al. (1995) J Biol Chem 270 (50) : 29781-29787.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_000557

Uniprot Accession Number

P12314

WB Description

The recombinant protein (20ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FCGR1A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of FCGR1A in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .Flow cytometry analysis of FCGR1A in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {2004010267727240.jpg}

IF Description

ICC/IF analysis of FCGR1A in HeLa cells. The cell was stained with ATGA0324 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . ICC/IF analysis of FCGR1A in Jurkat cells. The cell was stained with ATGA0324 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010267583857.jpg}