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Human Glutathione Reductase/GSR antibody

Glutathione reductase (GR) also known as glutathione-disulfide reductase (GSR) is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. Glutathione reductase functions as dimeric disulfide oxidoreductase and utilizes an FAD prosthetic group and NADPH to reduce one mole of GSSG to two moles of GSH.

Product Specifications

Product Name Alternative

Glutathione reductase, GLuR, GRD1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GSR (43-522aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT11D10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Glutathione Reductase, GSR, Glutathione reductase, GLuR, GRD1, ATGA0323-10 µg, ATGA0323-20 µg, ATGA0323-50 µg, ATGA0323-100 µg, ATGA0323-250 µg, ATGA0323-500 µg, ATGA0323-1 mg, ATGA0323-10, ATGA0323-20, ATGA0323-50, ATGA0323-100, ATGA0323-250, ATGA0323-500, ATGA0323-1

References & Citations

Deponte M. (2013) Biochim Biophys Acta 1830 (5) : 3217-3266; ; Meister A. (1988) J Biol Chem 263 (33) : 17205-17208; ; Mannervik B. (1987) Biochem Soc Trans 15 (4) : 717-718

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Redox Proteins

NCBI Accession Number

NP_000628

Uniprot Accession Number

P00390

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSR antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human GSR protein;

FACS Description

Flow cytometry analysis of GSR in Jurkat cells. The cell was stained with ATGA0323 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of GSR in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .
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