Human NKp44/NCR2 antibody

NKp44 (NCR2) is a member of the natural cytotoxicity receptor (NCR) family that is expressed on activated human NK cells. NKp44 displays a single extracellular Ig-like V domain and a transmembrane portion containing the charged residue (Lysine), likely involved in the association with KARAP/DAP12 molecules.

Product Specifications

Product Name Alternative

Natural cytotoxicity triggering receptor 2 isoform 1, CD336, LY95, NK-p44 NKP44, ANK0501

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NKp44 (19-130aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1G6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NKp44, NCR2, Natural cytotoxicity triggering receptor 2 isoform 1, CD336, LY95, NK-p44 NKP44, ATGA0322-10 µg, ATGA0322-20 µg, ATGA0322-50 µg, ATGA0322-100 µg, ATGA0322-250 µg, ATGA0322-500 µg, ATGA0322-1 mg, ATGA0322-10, ATGA0322-20, ATGA0322-50, ATGA0322-100, ATGA0322-250, ATGA0322-500, ATGA0322-1

References & Citations

Biassoni. R., et al., (2003) J Cell Mol Med 7: 376-387.; ; Kerry. S.C., et al., (2004) Journal of Immunology 172: 899-906.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_004819

Uniprot Accession Number

O95944

WB Description

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp44/NCR2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human NKp30 protein; ; Lane 2.: Recombinant human NKp44 protein; ; Lane 3.: Recombinant human NKp46 protein;

FACS Description

Flow cytometry analysis of NKp44/NCR2 in Jurkat cells. The cell was stained with ATGA0322 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of NKp44/NCR2 in Jurkat cells. The cell was stained with ATGA0322 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .