Human GRP78/HSPA5 antibody

Binding immunoglobulin protein (BIP) also known as 78kDa glucose-regulated protein (GRP-78) or heat shock 70kDa protein 5 (HSPA5) is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BIP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. BIP is an abundant protein under all growth conditions, but its synthesis is markedly induced under conditions that lead to the accumulation of unfolded polypeptides in the ER.

Product Specifications

Product Name Alternative

Heat shock protein family A member 5, Heat shock 70kD protein 5, HSP70 family protein 5, Glucose-regulated protein 78kD, Binding-immunoglobulin protein, BiP, Endoplasmic reticulum chaperone BiP, Glucose-regulated protein 78kDa, Immunoglobulin heavy chain-binding protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BIP (20-650aa) purified from Baculo virus

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT3D2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GRP78, HSPA5, Binding immunoglobulin protein, HSPA5, heat shock 70kDa protein 5, GRP78, glucose-regulated protein 78kD, MIF2, ATGA0320-10 µg, ATGA0320-20 µg, ATGA0320-50 µg, ATGA0320-100 µg, ATGA0320-250 µg, ATGA0320-500 µg, ATGA0320-1 mg, ATGA0320-10, ATGA0320-20, ATGA0320-50, ATGA0320-100, ATGA0320-250, ATGA0320-500, ATGA0320-1

References & Citations

Ting. J., et al. (1988) DNA 7 (4) : 275-86.; ; Hendershot. L.M., et al. (1994) Genomics 20 (2) : 281-284.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_005338

Uniprot Accession Number

P11021

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP78/HSPA5 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: MCF7 cell lysate;

IF Description

ICC/IF analysis of GRP78/HSPA5 in HeLa cells. The cell was stained with ATGA0320 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of GRP78/HSPA5 in Hep3B cells. The cell was stained with ATGA0320 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0320-IF2.jpg}