What is the catalog number for Human Bub3 antibody?

The catalog number for Human Bub3 antibody is ATGA0319-100.

What sizes are available for Human Bub3 antibody?

Human Bub3 antibody is available in the following size(s): 100 µL.

Is Human Bub3 antibody in stock?

Human Bub3 antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Human Bub3 antibody

BUB3 (budding uninhibited by benzimidazoles 3 homolog) is a conserved component of the mitotic spindle assembly complex (MCC) . It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments.

Product Specifications

Product Name Alternative

Mitotic checkpoint protein BuB3, BuB3L, hBuB3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BUB3 (1-328aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2H6

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Bub3, Mitotic checkpoint protein BuB3, BuB3L, hBuB3, ATGA0319-10 µg, ATGA0319-20 µg, ATGA0319-50 µg, ATGA0319-100 µg, ATGA0319-250 µg, ATGA0319-500 µg, ATGA0319-1 mg, ATGA0319-10, ATGA0319-20, ATGA0319-50, ATGA0319-100, ATGA0319-250, ATGA0319-500, ATGA0319-1

References & Citations

Loqarinho. E., et al. (2008) Mol Biol Cell 19 (4) : 1798-1813; ; Warren. C.D., et al. (2002) Mol Biol Cell 13 (9) : 3029-3041; ; Taylor. S.S., et al. (1998) J Cell Biol 142 (1) : 1-11

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000

Scientific Category

Cell Cycle

NCBI Accession Number

NP_004716

Uniprot Accession Number

O43684

WB Description

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.