Human CACYBP antibody

CACYBP (Calcyclin-binding protein) is primarily a nuclear protein that contains one CS domain and one SGS domain. CACYBP is believed to be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It most likely serves as a molecular bridge in ubiquitin E3 complexes. It also participates in the ubiquitin-mediated degradation of b-catenin. CACYBP is thought to be a potential inhibitor of cell growth and invasion in the gastric cancer cell through its effects on b-catenin protein expression and transcriptional activation of TCF/LEF.

Product Specifications

Product Name Alternative

Clcyclin-binding protein, GIG5, PNAS-107, RP1-102G20.6, S100A6BP, SIP, Clcyclin-binding protein CACYBP, Calcyclin binding protein, GIG 5, Growth inhibiting gene 5, Growth inhibiting gene 5 protein, hCacyBP, MGC87971, PNAS 107, PNAS107, RP1 102G20.6, S100A6 binding protein.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CACYBP (1-185aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT3G8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CACYBP, Clcyclin-binding protein, GIG5, PNAS-107, RP1-102G20.6, S100A6BP, SIP, Clcyclin-binding protein CACYBP, Calcyclin binding protein, GIG 5, Growth inhibiting gene 5, Growth inhibiting gene 5 protein, hCacyBP, MGC87971, PNAS 107, PNAS107, RP1 102G20.6, S100A6 binding protein., ATGA0316-10 µg, ATGA0316-20 µg, ATGA0316-50 µg, ATGA0316-100 µg, ATGA0316-250 µg, ATGA0316-500 µg, ATGA0316-1 mg, ATGA0316-10, ATGA0316-20, ATGA0316-50, ATGA0316-100, ATGA0316-250, ATGA0316-500, ATGA0316-1

References & Citations

Ghosh. D., et al. (2013) Mol Cell Proteomics 12 (7) : 1865-1880; ; Rines. A.K., et al. (2012) FASEB 26 (11) : 4685-4695; ; Ning. X., et al. (2007) Mol Cancer Res 5 (12) : 1254-1262

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001007215

Uniprot Accession Number

Q9HB71

WB Description

The cell lysates of HeLa, 293T, Jurkat and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CACYBP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of CACYBP in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CACYBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0316-IF1b.jpg}