What is the catalog number for Human ASF1B antibody?

The catalog number for Human ASF1B antibody is ATGA0315-100.

What sizes are available for Human ASF1B antibody?

Human ASF1B antibody is available in the following size(s): 100 µL.

Is Human ASF1B antibody in stock?

Human ASF1B antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Human ASF1B antibody

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. Delivering histone H3/H4 dimers to CAF-1 or HIRA histone deposition complexes to facilitate replication-coupled and replication-independent nucleosome assembly on DNA. Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to replicative stress.

Product Specifications

Product Name Alternative

ASF1 anti-silencing function 1 homolog B (S.cerevisiae), CIA-II

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ASF1B (1-202aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1D4

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ASF1B, ASF1 anti-silencing function 1 homolog B (S.cerevisiae), CIA-II, ATGA0315-10 µg, ATGA0315-20 µg, ATGA0315-50 µg, ATGA0315-100 µg, ATGA0315-250 µg, ATGA0315-500 µg, ATGA0315-1 mg, ATGA0315-10, ATGA0315-20, ATGA0315-50, ATGA0315-100, ATGA0315-250, ATGA0315-500, ATGA0315-1

References & Citations

Corpet A., et al. (2011) EMBO J 30:480-493; ; Mousson F., et al. (2007) Chromosoma 116:79-93; ; Hayashi R., et al. (2007) DNA Cell Biol 126:91-99

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_060624

Uniprot Accession Number

Q9NVP2

WB Description

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ASF1B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: ASF1B transfected 293T cell lysate