What is the catalog number for Human IL-6 antibody?

The catalog number for Human IL-6 antibody is ATGA0313-100.

What sizes are available for Human IL-6 antibody?

Human IL-6 antibody is available in the following size(s): 100 µL.

Is Human IL-6 antibody in stock?

Human IL-6 antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Human IL-6 antibody

Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length that plays critical roles in host defense, immune response and hematopoiesis. interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial and other cells. IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-Beta, is important for developing Th17 responses. The IL-6 receptor is a trimeric complex composed of an IL-6-specific a chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.

Product Specifications

Product Name Alternative

Interleukin-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IFNB2, HGF, HSF

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Interleukin 6 (30-212aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1F10

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

VLAB, VLA-4 subunit bet, VLA4 subunit bet, VLA 4 subunit bet, MSK12, MSK 12, MDF2, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta1, Integrin beta 1, IL-6, IL6, IL 6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, GP IIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0313-10 µg, ATGA0313-20 µg, ATGA0313-50 µg, ATGA0313-100 µg, ATGA0313-250 µg, ATGA0313-500 µg, ATGA0313-1 mg, ATGA0313-010, ATGA0313-020, ATGA0313-050, ATGA0313-100, ATGA0313-250, ATGA0313-500, ATGA0313-01M

References & Citations

Heinrich P.C., et al. (1998) Biochem J 334: 297-314; ; Heinrich P.C., et al. (1998) Z Ernahrungswiss 37: 43-49; ; Jones S.A. (2005) J Immunol 175: 3463-3468

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000591

Uniprot Accession Number

P05231

WB Description

The Human IL6 recombinant protein (10ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Interleukin 6 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.