Human 14-3-3 gamma antibody

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, Beta, Gamma, Epsilon, Delta, Zeta, Tau and Eta that have been identified in mammals. The 14-3-3 gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Product Specifications

Product Name Alternative

YWHAG, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma N-terminally processed, protein phosphatase 1, regulatory subunit 170, PPP1R170, 14-3-3γ

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4B9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

1-4-3-3g, 1433 gamma, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein gamma polypeptide, YWHAG, 14-3-3 gamma, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, gamma polypeptide 14 3 3 protein gamma, 14 3 3 protein gamma subtype, 14 3 3gamma, 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide, KCIP 1, KCIP1, Protein kinase C inhibitor protein 1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide., ATGA0312-10 µg, ATGA0312-20 µg, ATGA0312-50 µg, ATGA0312-100 µg, ATGA0312-250 µg, ATGA0312-500 µg, ATGA0312-1 mg, ATGA0312-10, ATGA0312-20, ATGA0312-50, ATGA0312-100, ATGA0312-250, ATGA0312-500, ATGA0312-1

References & Citations

Takagaki Y. and Manley J.L. (1992) J Biol Chem 267: 23471-23474; ; Takagaki Y. and Manley J.L. (2000) Mol Cell Biol 20: 1515-1525; ; Kleiman F.E. and Manley J.L. (1999) Science 285: 1576-1579

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036611

Uniprot Accession Number

P61981

WB Description

The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human YWHAZ ; ; Lane 2.: Recombinant Human YWHAB ; ; Lane 3.: Recombinant Human YWHAE ; ; Lane 4.: Recombinant Human YWHAH ; ; Lane 5.: Recombinant Human YWHAG ; ; Lane 6.: Recombinant Human SFN ; ; Lane 7.: Recombinant Human YWHAQ {ATGA0312-WB2.jpg} The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: A431 cell lysate ; ; Lane 3.: K562 cell lysate ; ; Lane 4.: NIH3T3 cell lysate {ATGA0312-WB3.jpg}

IF Description

ICC/IF analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .