Human IGF-1 antibody

Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. IGF-1 has also been referred to as a Sulfation factor and its effects were termed nonsuppressible insulin-like activity (NSILA) in the 1970s. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin, is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7, 649 daltons.

Product Specifications

Product Name Alternative

IBP1, IGF-1, IGFI, IGF-IA, IGF-IB, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor-1 IGF IA, Mechano growth factor, MGF, Somatomedin C

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IGF1 (49-118aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT6F8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

IBP1, IGF-IB, IGF IB, IGF1, IGF-1, IGFI, IGF-I, IGFIA, IGF-IA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor-1, Insulin-like growth factor-1 IGF IA, Mechano growth factor, MGF, Somatomedin C, Somatomedin-C, ATGA0311-10 µg, ATGA0311-20 µg, ATGA0311-50 µg, ATGA0311-100 µg, ATGA0311-250 µg, ATGA0311-500 µg, ATGA0311-1 mg, ATGA0311-010, ATGA0311-020, ATGA0311-050, ATGA0311-100, ATGA0311-250, ATGA0311-500, ATGA0311-01M

References & Citations

Hoppener J.W., et al. (1985) Hum Genet 69 (2) : 157-160; ; Jansen M., et al. (1983) Nature 306 (5943) : 609-611; ; Salmon W., et al. (1957) J Lab Clin Med 49 (6) : 825-836 ; ; Keating G.M., (2008) BioDrugs 22 (3) : 177-188; ; Rinderknecht E., et al. (1978) J Biol Chem 253 (8) : 2769-2776

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000609

Uniprot Accession Number

P05019

WB Description

The human IGF1 recombinant protein (12.5ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: IGF1 transfected 293T cell lysate {ATGA0311-WB2.jpg}

IF Description

ICC/IF analysis of IGF1 in PC3 cells. The cell was stained with ATGA0311 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .