Human HIP/ST13 antibody

HIP (Hsc70-interacting protein), also known as ST13, is a co-chaperone to the major heat shock proteins, HSP70 and HSP90, and appears in early receptor complexes. Through mutual binding to both HSP70 and HSP90, Hip functions as an adaptor that can integrate HSP70 and HSP90 interactions. Also, Hip has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones.

Product Specifications

Product Name Alternative

Hsc70-interacting protein, ST13, HOP, SNC6, FAM10A1, Hsc70-interacting protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human HIP/ST13 (1-369aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT5C6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ST13, Hsc70-interacting protein, ST13, HOP, SNC6, FAM10A1, Hsc70-interacting protein, ATGA0309-10 µg, ATGA0309-20 µg, ATGA0309-50 µg, ATGA0309-100 µg, ATGA0309-250 µg, ATGA0309-500 µg, ATGA0309-1 mg, ATGA0309-10, ATGA0309-20, ATGA0309-50, ATGA0309-100, ATGA0309-250, ATGA0309-500, ATGA0309-1

References & Citations

Nelson GM, et al., (2004) Mol Endocrinol.18 (7) : 1620-30; ; Johnson BD, et al., (1998) J Biol Chem. 273 (6) :3679-86

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_003923

Uniprot Accession Number

P50502

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ST13 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: SW480 cell lysate ; ; Lane 4.: Jurkat cell lysate ; ; Lane 5.: K562 cell lysate ; ; Lane 6.: LnCap cell lysate ; ; Lane 7.: HeLa cell lysate ; ; Lane 8.: PC3 cell lysate

FACS Description

Flow cytometry analysis of ST13 in 293T cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of ST13 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ATGA0309-FC2.jpg}

IF Description

ICC/IF analysis of ST13 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of ST13 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0309-IF2.jpg} ICC/IF analysis of ST13 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0309-IF3.jpg}