Human Cathepsin Z antibody

CTSZ (Cathepsin Z), also known as Cathepsin X and Cathepsin P, is 303 amino acid protein. It is lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity. This gene is widely expressed in cancer cell lines, primary tumors. CTSZ may be involved in tumor progression. And, CTSZ plays a role in normal intracellular protein degradation

Product Specifications

Product Name Alternative

Carboxypeptidase LB, Cathepsin B2, Cathepsin IV, Cathepsin P, Cathepsin X, Cathepsin Y, Cathepsin Z, Cathepsin Z preproprotein, Cathepsin Z1, CatX, CTSP, CTSX, CTSZ, Cysteine-type carboxypeptidase, Lysosomal carboxypeptidase B

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CTSZ (62-303aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT6G11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Carboxypeptidase LB, Cathepsin B2, Cathepsin IV, Cathepsin P, Cathepsin X, Cathepsin X Z P, Cathepsin X, Z, P, Cathepsin X/Z/P, Cathepsin XZP, Cathepsin Y, Cathepsin Z, Cathepsin Z preproprotein, Cathepsin Z1, Cathepsin-IV, Cathepsin-X, Cathepsin-Y, Cathepsin-Z, Cathepsin-Z1, Cathepsin-B2, CatX, CTSP, CTSX, CTSZ, Cysteine-type carboxypeptidase, Lysosomal carboxypeptidase B, ATGA0304-10 µg, ATGA0304-20 µg, ATGA0304-50 µg, ATGA0304-100 µg, ATGA0304-250 µg, ATGA0304-500 µg, ATGA0304-1 mg, ATGA0304-010, ATGA0304-020, ATGA0304-050, ATGA0304-100, ATGA0304-250, ATGA0304-500, ATGA0304-01M

References & Citations

Pungercar. J., et al. (2000) Pflugers Arch 439: R119-R121; ; Pungercar. J. and Ivanovski. G. (2000) Pflugers Arch 439: R116-R118 ; ; Naegler. D.K., et al. (1999) Biochemistry 38: 12648-12654;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Scientific Category

Tags & Cell Markers

NCBI Accession Number

NP_001327

Uniprot Accession Number

Q9UBR2

WB Description

The cell lysates of HeLa (40ug) and SW480 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSZ antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSZ antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Recombinant Human CTSB ; ; Lane 2. : Recombinant Human CTSD ; ; Lane 3. : Recombinant Human CTSE ; ; Lane 4. : Recombinant Human CTSF ; ; Lane 5. : Recombinant Human CTSH ; ; Lane 6. : Recombinant Human CTSK ; ; Lane 7. : Recombinant Human CTSL ; ; Lane 8. : Recombinant Human CTSS ; ; Lane 9. : Recombinant Human CTSW ; ; Lane 10. : Recombinant Human CTSZ {ATGA0304-WB2.jpg}

IF Description

ICC/IF analysis of CTSZ in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTSZ antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .