Human Coactosin-like Protein 1/COTL1 antibody

COTL1 (coactosin-like 1), also known as CLP, is a 142 amino acid cytoplasmic and cytoskeletal protein that belongs to the actin-binding proteins ADF family and coactosin subfamily. COTL1 is first identified in slime mold and is found at highest levels in kidney, placenta, lung and peripheral blood leukocytes. COTL1 is one of many proteins that participate in regulation of Actin's cytoskeleton through a calcium dependent mechanism, but has not been shown to have a direct effect on Actin depolymerization.

Product Specifications

Product Name Alternative

Coactosin like F-actin binding protein 1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, CLP

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human COTL1 (1-142aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1D6

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CLP, Coactosin like F-actin binding protein 1, Coactosin like Protein1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, Coactosin-like protein, Coactosin-like Protein 1, Coactosin-like Protein1, COTL 1, COTL1, COTL-1, FLJ43657, MGC19733, ATGA0300-10 µg, ATGA0300-20 µg, ATGA0300-50 µg, ATGA0300-100 µg, ATGA0300-250 µg, ATGA0300-500 µg, ATGA0300-1 mg, ATGA0300-010, ATGA0300-020, ATGA0300-050, ATGA0300-100, ATGA0300-250, ATGA0300-500, ATGA0300-01M

References & Citations

Provost. P., et al. (2001) Biochem J 359 (1) : 255-263.; ; Dai. H., et al. (2006) Biochim Biophys Acta 1764 (11) : 1688-1700.; ; Esser. J., et al. (2010) Biochem J 425 (1) : 265-274.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_066972

Uniprot Accession Number

Q14019

WB Description

The cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Coactosin-like Protein 1/COTL1 Transfected 293T cell lysate; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Coactosin-like Protein 1/COTL1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: PC3 cell lysate; ; Lane 2.: A549 cell lysate; ; Lane 3.: U87MG cell lysate; ; Lane 4.: HeLa cell lysate; ; {ATGA0300-WB2.jpg}

FACS Description

Flow cytometry analysis of Coactosin-like Protein 1/COTL1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .